Photographer: Jeroen Oerlemans

prof. dr. G.L. (Garry) Corthals


  • Faculty of Science
    HIMS
  • Visiting address
    Science Park A
    Science Park 904  Amsterdam
    Room number: C2.251
  • Postal address:
    Postbus  94720
    1090 GS  Amsterdam
  • G.L.Corthals@uva.nl

Mass spectrometric research to study dynamic and system-wide changes in biomedicine. 

Mass spectrometry is central to most of the work we do because it enables the precise identity and quantity of proteins. MS instruments and have three distinct sections where technology development occurs: (i) sample preparation, including high resolution molecular separation and molecular ionisation, (ii) mass analysis in the mass spectrometer and finally (iii) data analysis, that deals with massive amounts of molecular information. 

There is a wide-scope of research activity we work on, from health and disease to forensics and biotechnology. Essentially any field if science that deals with molecular biosystems. Recently we have started new focus area with other groups at HIMS that deals with conservation science in the ' Science for Art' project. 

The work in our group focuses on the development of a variety of technologies embedded or allied to mass spectrometers, and the application of MS technology to a range of biomedical applications.

Our research focuses on four key areas:

  • Mass spectrometric tools and allied technologies aimed at sensitive and quantitative analysis of proteins and their PTMs
  • Separation sciences that can efficiently resolve complex molecular mixtures so their analysis is within the capacity of our analytical technology
  • Appication of new chemistries in biomedical applications to reveal molecular functional information of protein networks and protein complexes
  • Computational and statistical methods to assist in the efficient validation and understanding of proteome-scale analysis of biomolecular systems. 

Selected publications

 

2015

  • Nguyen EV, Imanishi SY, Haapaniemi P, Yadav A, Saloheimo M, Corthals GL, Pakula TM. Quantitative Site-Specific Phosphoproteomics of Trichoderma reesei Signaling Pathways upon Induction of Hydrolytic Enzyme Production. J Proteome Res. 2016 Feb 5;15(2):457-67. doi: 10.1021/acs.jproteome.5b00796. Epub 2016 Jan 14. PubMed PMID: 26689635.
  • Vuorijoki L, Isojärvi J, Kallio P, Kouvonen P, Aro EM, Corthals GL, Jones PR,  Muth-Pawlak D. Development of a Quantitative SRM-Based Proteomics Method to Study Iron Metabolism of Synechocystis sp. PCC 6803. J Proteome Res. 2016 Jan 4;15(1):266-79. doi: 10.1021/acs.jproteome.5b00800. Epub 2015 Dec 22. PubMed PMID: 26652789.
  • Vehmas AP, Adam M, Laajala TD, Kastenmüller G, Prehn C, Rozman J, Ohlsson C, Fuchs H, Hrabě de Angelis M, Gailus-Durner V, Elo LL, Aittokallio T, Adamski J, Corthals G, Poutanen M, Strauss L. Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse. J Proteomics. 2016 Feb 5;133:66-75. doi: 10.1016/j.jprot.2015.12.009. Epub 2015 Dec 9. PubMed PMID: 26691839.
  • Suomi T, Corthals GL, Nevalainen OS, Elo LL. Using Peptide-Level Proteomics Data for Detecting Differentially Expressed Proteins. J Proteome Res. 2015 Nov 6;14(11):4564-70. doi: 10.1021/acs.jproteome.5b00363. Epub 2015 Sep 29. PubMed PMID: 26380941.
  • Pursiheimo A, Vehmas AP, Afzal S, Suomi T, Chand T, Strauss L, Poutanen M, Rokka A, Corthals GL, Elo LL. Optimization of Statistical Methods Impact on Quantitative Proteomics Data. J Proteome Res. 2015 Oct 2;14(10):4118-26. doi: 10.1021/acs.jproteome.5b00183. Epub 2015 Sep 8. PubMed PMID: 26321463.
  • Pokharel YR, Saarela J, Szwajda A, Rupp C, Rokka A, Lal Kumar Karna S, Teittinen K, Corthals G, Kallioniemi O, Wennerberg K, Aittokallio T, Westermarck J. Relevance Rank Platform (RRP) for Functional Filtering of High Content Protein-Protein Interaction Data. Mol Cell Proteomics. 2015 Dec;14(12):3274-83. doi: 10.1074/mcp.M115.050773. Epub 2015 Oct 23. PubMed PMID: 26499835; PubMed Central PMCID: PMC4762622.
  • Kottahachchi D, Gooneratne L, Jayasekera A, Muth-Pawlak D, Moulder R, Imanishi SY, Ariyaratne A,  Rokka A, Corthals GL. Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide. EuPA Open Proteom. 2015 Jun;7:43-53.
  • Kauko O, Laajala TD, Jumppanen M, Hintsanen P, Suni V, Haapaniemi P, Corthals G, Aittokallio T, Westermarck J & Imanishi SY, Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling, Scientific Reports, 2015, Aug 17;5:13099 doi: 10.1038/srep13099. PubMed PMID: 26278961; PubMed Central PMCID: PMC4642524.
  • Virtakoivu R, Mai A, Mattila E, De Franceschi N, Imanishi SY, Corthals G, Kaukonen R, Saari M, Cheng F, Torvaldson E, Kosma VM, Mannermaa A, Muharram G, Gilles C, Eriksson J, Soini Y, Lorens JB, Ivaska J. Vimentin-ERK signaling uncouples Slug gene regulatory function. Cancer Res. 2015, Jun1;75(11):2349-62. doi: 10.1158/0008-5472.CAN-14-2842. Epub 2015 Apr 8. PubMed PMID: 25855378.
  • Wheelock ÅM, Paulson L, Litton JE; EuPA Biobank Initiative Group. The EuPA Biobank Initiative: Meeting the future challenges of biobanking in proteomics & systems medicine. J Proteomics. 2015 Sep 8;127(Pt B):414-6. PubMed PMID: 26216594.
  • Anttonen AK, Hilander T, Linnankivi T, Isohanni P, French RL, Liu Y, Simonovi M, Söll D, Somer M, Muth-Pawlak D, Corthals GL, Laari A, Ylikallio E, Lähde M, Valanne L, Lönnqvist T, Pihko H, Paetau A, Lehesjoki AE, Suomalainen A, and Tyynismaa H, Selenoprotein biosynthesis defect causes progressive encephalopathy with elevated lactate, Neurology, 2015, Jul28;85(4):306-15 doi: 10.1212/WNL.0000000000001787. PubMed PMID: 26115735; PubMed Central PMCID: PMC4520820.
  • Suni V, Imanishi SY, Maiolica A, Aebersold R, Corthals GL. Confident site localization using a simulated phosphopeptide spectral library. J Proteome Res. 2015 May 1;14(5):2348-59. doi:10.1021/acs.jproteome.5b00050. PubMed PMID: 25774671.
  •  McDonnell LA, Römpp A, Balluff B, Heeren RM, Albar JP, Andrén PE, Corthals GL, Walch A, Stoeckli M. Discussion point: reporting guidelines for mass spectrometry imaging. Anal Bioanal Chem. 2015 Mar;407(8):2035-45. doi: 10.1007/s00216-014-8322-6. PubMed PMID: 25432304.

2014

  • McDonnell LA, Römpp A, Balluff B, Heeren RM, Albar JP, Andrén PE, Corthals GL, Walch A, Stoeckli M. Discussion point: reporting guidelines for mass spectrometry imaging. Anal Bioanal Chem. 2014 Nov 29. 
  • Oksanen A, Aittomäki S, Jankovic D, Ortutay Z, Pulkkinen K, Hämäläinen S, Rokka A, Corthals GL, Watford WT, Junttila I, O'Shea JJ, Pesu M. Proprotein Convertase FURIN Constrains Th2 Differentiation and Is Critical for Host Resistance against Toxoplasma gondii. J Immunol. 2014 Oct 29. 
  • Konert G, Trotta A, Kouvonen P, Rahikainen M, Durian G, Blokhina O, Fagerstedt K, Muth D, Corthals GL, Kangasjärvi S.  Protein phosphatase 2A (PP2A) regulatory subunit B'γ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana. New Phytol. 2014 Oct 13.
  • Vehmas AP, Muth-Pawlak D, Huhtinen K, Saloniemi-Heinonen T, Jaakkola K, Laajala TD, Kaprio H, Suvitie PA, Aittokallio T, Siitari H, Perheentupa A, Poutanen M, Corthals GL.  Ovarian Endometriosis Signatures Established through Discovery and Directed Mass Spectrometry Analysis. J Proteome Res. 2014 Aug 20. Kannaste O, Suomi T, Salmi J, Uusipaikka E, Nevalainen O, Corthals GL.  Cross-correlation of spectral count ranking to validate quantitative proteome measurements. J Proteome Res. 2014 Apr 4;13(4):1957-68. 
  • Kannaste O, Suomi T, Salmi J, Uusipaikka E, Nevalainen O, Corthals GL., Cross-correlation of spectral count ranking to validate quantitative proteome measurements. J Proteome Res. 2014 Apr 4;13(4):1957-68.
  • McDonnell LA, Walch A, Stoeckli M, Corthals GL.  MSiMass list: a public database of identifications for protein MALDI MS imaging. J Proteome Res. 2014 Feb 7;13(2):1138-42. 

2013

  • Westermarck J, Ivaska J, Corthals GL.  Identification of protein interactions involved in cellular signaling. Mol Cell Proteomics. 2013 Jul;12(7):1752-63. 
  • Santos HM, Kouvonen P, Capelo JL, Corthals GL.  On-target ultrasonic digestion of proteins. Proteomics. 2013 May;13(9):1423-7. 
  • Koch S, Scifo E, Rokka A, Trippner P, Lindfors M, Korhonen R, Corthals GL, Virtanen I, Lalowski M, Tyynelä J.  Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain. Neurobiol Dis. 2013 Feb;50:107-19. 
  • Velasquez EV, Ríos M, Ortiz ME, 1Carlos L, Nuñez E, Abramovic D, Orge F, Rokka A, Oliva B, Orellana R, Villalon M, Moreno R, Tesone M, Corthals GL, Croxatto H, Parborell F, Owen G. Concanavalin-A induces granulosa cell death and inhibits FSH mediated follicular growth and ovarian maturation in female rats. Endocrinology. 2013 May;154(5):1885-96

2012

  • Perdomo MF, Hosia W, Jejcic A, Corthals GL, Vahlne A.  Human serum protein enhances HIV-1 replication and up-regulates the transcription factor AP-1. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17639-44. 
  • McDonnell L, Andrén PE, Corthals GL.  Imaging mass spectrometry: a user’s guide to a new technique for biological and biomedical research. J Proteomics. 2012 Aug 30;75(16):4881-2. 
  • McDonnell LA, Heeren RM, Andrén PE, Stoeckli M, Corthals GL.  Going forward: Increasing the accessibility of imaging mass spectrometry. J Proteomics. 2012 Aug 30;75(16):5113-21. 
  • Santos HM, Kouvonen P, Capelo JL, Corthals GL.  Isotopic labelling of peptides in tissues enhances mass spectrometric profiling. Rapid Commun Mass Spectrom. 2012 Feb 15;26(3):254-62. 

2011

  • Legrain P, Aebersold R, Archakov A, Bairoch A, Bala K, Beretta L, Bergeron J, Borchers C, Corthals GL, Costello CE, Deutsch EW, Domon B, Hancock W, He F, Hochstrasser D, Marko-Varga G, Salekdeh GH, Sechi S, Snyder M, Srivastava S, Uhlen M, Hu CH, Yamamoto T, Paik YK, Omenn GS.  The human proteome project: Current state and future direction. Mol Cell Proteomics. 2011 Apr 29.  
  • Rodrigues AJ, Neves-Carvalho A, Teixeira-Castro A, Rokka A, Corthals G, Logarinho E, Maciel P.  Absence of ataxin-3 leads to enhanced stress response in C. elegans. PLoS One. 2011 Apr 19;6(4):e18512. 
  • Kouvonen P, Rainio EM, Suni V, Koskinen P, Corthals GL.  Enrichment and sequencing of phosphopeptides on indium tin oxide coated glass slides. Mol Biosyst. 2011 Jun;7(6):1828-37. 
  • Leivonen SK, Rokka A, Ostling P, Kohonen P, Corthals GL, Kallioniemi O, Perälä M.  Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact. Mol Cell Proteomics. 2011 Jul;10(7):M110.005322. 

2010

  • Kouvonen P, Rainio EM, Suni V, Koskinen P, Corthals GL.  Data  combination from multiple matrix-assisted laser desorption/ionization (MALDI) matrices: opportunities and limitations for MALDI analysis. Rapid Commun Mass Spectrom. 2010 Dec 15;24(23):3493-5. 
  • Medina-Aunon JA, Paradela A, Macht M, Thiele H, Corthals G, Albar JP.  Protein Information and Knowledge Extractor: Discovering biological information from proteomics data. Proteomics. 2010 Sep;10(18):3262-71. 
  • do Carmo Costa M, Bajanca F, Rodrigues AJ, Tomé RJ, Corthals G, Macedo-Ribeiro S, Paulson HL, Logarinho E, Maciel P.  Ataxin-3 plays a role in mouse myogenic differentiation through regulation of integrin subunit levels. PLoS One. 2010 Jul 23;5(7):e11728. 
  • McDonnell LA, Corthals GL, Willems SM, van Remoortere A, van Zeijl RJ, Deelder AM.  Peptide and protein imaging mass spectrometry in cancer research. J Proteomics. 2010 Sep 10;73(10):1921-44. 
  • Moulder R, Lönnberg T, Elo LL, Filén JJ, Rainio E, Corthals G, Oresic M, Nyman TA, Aittokallio T, Lahesmaa R.  Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation. Mol Cell Proteomics. 2010 Sep;9(9):1937-53. 
  • Imanishi SY, Kouvonen P, Smått JH, Heikkilä M, Peuhu E, Mikhailov A, Ritala M, Lindén M, Corthals GL, Eriksson JE.  Phosphopeptide enrichment with stable spatial coordination on a titanium dioxide coated glass slide. Rapid Commun Mass  Spectrom. 2009 Dec;23(23):3661-7.
  • Mutka AL, Haapanen A, Käkelä R, Lindfors M, Wright AK, Inkinen T, Hermansson M, Rokka A, Corthals G, Jauhiainen M, Gillingwater TH, Ikonen E, Tyynelä J.  Murine cathepsin D deficiency is associated with dysmyelination/myelin disruption and accumulation of cholesteryl esters in the brain. J Neurochem. 2010 Jan;112(1):193-203. 

2009

  • Rodrigues AJ, Neves-Carvalho A, Ferro A, Rokka A, Corthals G, Logarinho E, Maciel P.  ATX-3, CDC-48 and UBXN-5: a new trimolecular complex in Caenorhabditis  elegans. Biochem Biophys Res Commun. 2009 Sep 4;386(4):575-81. 
  • Kouvonen P, McDonnell LA, Heeren RM, Corthals GL.  Nitromatrix provides improved LC-MALDI signals and more protein identifications. Proteomics. 2009 Mar;9(6):1662-71.

2008

  • Mattinen L, Somervuo P, Nykyri J, Nissinen R, Kouvonen P, Corthals G, Auvinen P, Aittamaa M, Valkonen JP, Pirhonen M.  Microarray profiling of host-extract-induced genes and characterization of the type VI secretion cluster  in the potato pathogen Pectobacterium atrosepticum. Microbiology. 2008 Aug;154(Pt 8):2387-96. 
  • Buggiotti L, Primmer CR, Kouvonen P, Bures S, Corthals GL, Leder EH.  Identification of differentially expressed proteins in Ficedula flycatchers. Proteomics. 2008 May;8(10):2150-3.

2007

  • Imanishi SY, Kochin V, Ferraris SE, de Thonel A, Pallari HM, Corthals GL, Eriksson JE.  Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by microLC-ESI-Q-TOF MS/MS. Mol Cell Proteomics. 2007 Aug;6(8):1380-91.
  • Corthals GL, Wasinger VC, Goodlett DR.  Offline Micro-IMAC Enrichment of Phosphoproteins. CSH Protoc. 2007 Nov 1;2007:pdb.prot4624.

New positions: 

New and enthusiastic researchers are always welcome. If you have a background in MS and are inteersted in joinging the group by applying for funding then please contact Garry Corthals. We are always looking for experts in quantitative MS with a combination of skill and flexibility; people who can collaborate, adapt quickly, are enjoyable company and have the drive to get things done.

 

Janurary 2015

  • Petra Jansen starts in group

 

December 2014

  • New Triple Tof installed and training commenced. 

 

Mid 2014

  • Garry Corthals commences as new chair in Biomolecular Systems Analytics

  • Michelle Camenzulli and Garry Corthals form group in Biomolecular Systems Analytics

2017

  • Wouters, B., Dapic, I., Valkenburg, T. S. E., Wouters, S., Niezen, L., Eeltink, S., ... Schoenmakers, P. J. (2017). A cyclic-olefin-copolymer microfluidic immobilized-enzyme reactor for rapid digestion of proteins from dried blood spots. Journal of Chromatography A, 1491, 36-42. DOI: 10.1016/j.chroma.2017.01.078 [details]

2016

  • Canals, F., Elortza, F., Paradela, A., Corthals, G., Camenzuli, M., Muñoz, A., ... Corrales, F. J. (2016). The EuPA Standardization Initiative. EuPA Open Proteomics, 11, 31-32. DOI: 10.1016/j.euprot.2016.03.007 [details] [PDF]
  • Kolmeder, C. A., Salojarvi, J., Ritari, J., de Been, M., Raes, J., Falony, G., ... de Vos, W. M. (2016). Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults. PLoS One, 11(4). DOI: 10.1371/journal.pone.0153294 [details] [PDF]
  • Nguyen, E. V., Imanishi, S. Y., Haapaniemi, P., Yadav, A., Saloheimo, M., Corthals, G. L., & Pakula, T. M. (2016). Quantitative site-specific phosphoproteomics of Trichoderma reesei signaling pathways upon induction of hydrolytic enzyme production. Journal of Proteome Research, 15(2), 457-467. DOI: 10.1021/acs.jproteome.5b00796 [details]
  • Santio, N. M., Landor, S. K. -J., Vahtera, L., Yla-Pelto, J., Paloniemi, E., Imanishi, S. Y., ... Koskinen, P. J. (2016). Phosphorylation of Notch1 by Pim kinases promotes oncogenic signaling in breast and prostate cancer cells. OncoTarget, 7(28), 43220-43238. DOI: 10.18632/oncotarget.9215 [details]
  • Soderholm, S., Kainov, D. E., Ohman, T., Denisova, O. V., Schepens, B., Kulesskiy, E., ... Nyman, T. A. (2016). Phosphoproteomics to Characterize Host Response During Influenza A Virus Infection of Human Macrophages. Molecular & Cellular Proteomics, 15(10), 3203-3219. DOI: 10.1074/mcp.M116.057984 [details]
  • Vehmas, A. P., Adam, M., Laajala, T. D., Kastenmüller, G., Prehn, C., Rozman, J., ... Strauss, L. (2016). Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse. Journal of Proteomics, 133, 66-75. DOI: 10.1016/j.jprot.2015.12.009 [details]
  • Vuorijoki, L., Isojärvi, J., Kallio, P., Kouvonen, P., Aro, E. M., Corthals, G. L., ... Muth-Pawlak, D. (2016). Development of a quantitative SRM-based proteomics method to study iron metabolism of Synechocystis sp PCC 6803. Journal of Proteome Research, 15(1), 266-279. DOI: 10.1021/acs.jproteome.5b00800 [details]

2015

  • Kauko, O., Laajala, T. D., Jumppanen, M., Hintsanen, P., Suni, V., Haapaniemi, P., ... Imanishi, S. Y. (2015). Label-free quantitative phosphoproteomics with novel pairwise abundance normalization reveals synergistic RAS and CIP2A signaling. Scientific Reports, 5, [13099]. DOI: 10.1038/srep13099 [details / files]
  • McDonnell, L. A., Römpp, A., Balluff, B., Heeren, R. M. A., Albar, J. P., Andrén, P. E., ... Stoeckli, M. (2015). Discussion point: reporting guidelines for mass spectrometry imaging. Analytical and Bioanalytical Chemistry, 407(8), 2035-2045. DOI: 10.1007/s00216-014-8322-6 [details]
  • Pursiheimo, A., Vehmas, A. P., Afzal, S., Suomi, T., Chand, T., Strauss, L., ... Elo, L. L. (2015). Optimization of statistical methods impact on quantitative proteomics data. Journal of Proteome Research, 14(10), 4118-4126. DOI: 10.1021/acs.jproteome.5b00183 [details]
  • Suni, V., Imanishi, S. Y., Maiolica, A., Aebersold, R., & Corthals, G. L. (2015). Confident Site Localization Using a Simulated Phosphopeptide Spectral Library. Journal of Proteome Research, 14(5), 2348-2359. DOI: 10.1021/acs.jproteome.5b00050 [details]
  • Suomi, T., Corthals, G. L., Nevalainen, O. S., & Elo, L. L. (2015). Using peptide-level proteomics data for detecting differentially expressed proteins. Journal of Proteome Research, 14(11), 4564-4570. DOI: 10.1021/acs.jproteome.5b00363 [details]

2014

  • Kannaste, O., Suomi, T., Salmi, J., Uusipaikka, E., Nevalainen, O., & Corthals, G. L. (2014). Cross-Correlation of Spectral Count Ranking to Validate Quantitative Proteome Measurements. Journal of Proteome Research, 13(4), 1957-1968. DOI: 10.1021/pr401096z [details]
This list of publications is extracted from the UvA-Current Research Information System. Questions? Ask the library or the Pure staff of your faculty / institute. Log in to Pure to edit your publications.
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