Speaker: Professor Yves Mély (Director of Laboratory of Bioimaging and Pathologies, University of Strasbourg, France)
|Date||7 December 2018|
Science Park 904, room B0.206
Yves Mély is Professor of biophysics at the University of Strasbourg. Since 1999, he is team leader and since 2009, he is head of the Laboratory of Bioimaging and Pathologies. His research activities focus on 1) the development of innovative environmentally-sensitive fluorescence probes for labeling of nucleic acids, proteins and membranes, and cutting-edge fluorescence techniques (quantitative and high resolution microscopy techniques) and 2) the application of these techniques and probes to monitor biomolecular interactions, mainly using viral and epigenetic proteins. He has published about 300 international publications, and has given about 130 invited lectures. He has directed 36 PhD thesis. He was a von Humboldt fellow for a sabbatical at the Max Planck Institute of Biophysics (Frankfurt) in 1987 and a senior visiting scientist at Riken in 2015. He received the Gregorio Weber award for excellence in fluorescence in 2017. He is Honorary Doctor of the National Taras Shevchenko University of Kiev and senior member of the Institut Universitaire de France. He is the chair of the MAF conference series and co-chair of the FB3 conference series. He is the co-founder and editor in chief of Methods and Applications in Fluorescence.
Environmentally-sensitive fluorescent probes provide crucial information on the properties of their surroundings through their photophysical properties. They are thus highly used to label biomolecules or cellular compartments in order to address a large range of biologically relevant questions. In this context, we developed and applied a number of these probes, including two color probes of the 3-hydroxychromone (3HC) family, characterized by two well-separated emission bands resulting from an excited state intramolecular proton transfer. These probes were used to label lipid membranes in order to characterize their physico-chemical properties, but also to monitor cell apoptosis. We also developed probes for labeling peptides and nucleic acids, in order to sensitively monitor the interaction of the labeled biomolecules with a large range of ligands. These probes were also used as building blocks to design amino acid and nucleotide surrogates that minimally perturb the structure and functions of the labeled proteins or nucleic acids. These surrogates were successfully used to monitor the dynamics of conformational transitions and localized events in nucleic acids, providing key information on the mechanism of viral and epigenetic proteins.
This lecture is sponsored by the John van Geuns Foundation.