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In a paper just published in Chemical Reviews, Noor Abdulhussain, Suhas Nawada and Peter Schoenmakers of the HIMS research group Analytical Chemistry provide an overview of current and future developments in three-dimensional separations in chromatography, which are crucial for the analysis of highly complex mixtures. The article is part of the 'Frontiers of Analytical Science' special issue of Chemical Reviews.

Graphical abstract
Image: HIMS.


Separation and characterization of complex mixtures are of crucial importance in many fields, where extremely high separation power is required. Three-dimensional separation techniques can offer a path toward achieving high peak capacities. In this review, online three-dimensional separation systems are discussed, including three-dimensional gas chromatography, and hyphenated combinations of two-dimensional gas chromatography with liquid chromatography or supercritical fluid chromatography. Online comprehensive two-dimensional liquid chromatography provides detailed information on complex samples and the need for higher peak capacities is pushing researchers toward online three-dimensional liquid chromatography. In this review, an overview of the various combinations is provided and we discuss and compare their potential performance, advantages, perspectives, and results obtained during the most recent 10–15 years. Finally, the review will discuss a novel approach of spatial three-dimensional liquid separation to increase peak capacity.


Noor Abdulhussain, Suhas Nawada, and Peter Schoenmakers: Latest Trends on the Future of Three-Dimensional Separations in Chromatography. Chem. Rev. 2021, Publication Date April 20, 2021. DOI: 10.1021/acs.chemrev.0c01244