Dr. B.W.J. (Bob) Pirok
Faculty of Science
Van 't Hoff Institute for Molecular Sciences
Visiting address
- Science Park 904
- Room number: C2.258
Postal address
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Postbus 94157
1090 GD Amsterdam
Contact details
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Publications
- refereed (84)
- conference contributions (5)
- recognitions (44)
- supervised theses (7)
- doctoral thesis (1)
2025
- Pirok, B. W. J., & Schoenmakers, P. J. (2025). Analytical Separation Science. Royal Society of Chemistry. https://doi.org/10.1039/9781837674824 [details]
van Henten, G. B., Boelrijk, J., Kattenberg, C., Bos, T. S., Ensing, B., Forré, P., & Pirok, B. W. J. (2025). Comparison of optimization algorithms for automated method development of gradient profiles. Journal of Chromatography A, 1742, Article 465626. https://doi.org/10.1016/j.chroma.2024.465626 [details]- van den Hurk, R. S., Lemmink, I. B., van Schaik, J. R., Hulsbergen, A. W. C., Peters, R. A. H., Pirok, B. W. J., & van Asten, A. C. (2025). Characterizing nitrocellulose by nitration degree and molecular weight: A user-friendly yet powerful forensic comparison method for smokeless powders. Forensic Chemistry, 42, Article 100637. https://doi.org/10.1016/j.forc.2024.100637 [details]
- van den Hurk, R. S., Mengerink, Y., Peters, R. A. H., van Asten, A. C., Pirok, B. W. J., & Bos, T. S. (2025). Introducing an algorithm to accurately determine copolymer block-length distributions. Analytica Chimica Acta, 1354, Article 343990. https://doi.org/10.1016/j.aca.2025.343990
2024
- Boelrijk, J., Molenaar, S. R. A., Bos, T. S., Dahlseid, T. A., Ensing, B., Stoll, D. R., Forré, P., & Pirok, B. W. J. (2024). Enhancing LC×LC separations through multi-task Bayesian optimization. Journal of Chromatography A, 1726, Article 464941. https://doi.org/10.1016/j.chroma.2024.464941 [details]
- Bos, T. S., Pirok, B. W. J., Karlson, L., Schantz, S., Dahlseid, T. A., Stoll, D. R., & Somsen, G. W. (2024). Fingerprinting of hydroxy propyl methyl cellulose by comprehensive two-dimensional liquid chromatography-mass spectrometry of monomers resulting from acid hydrolysis. Journal of Chromatography A, 1722, Article 464874. https://doi.org/10.1016/j.chroma.2024.464874 [details]
- Milani, N. B. L., García-Cicourel, A. R., Blomberg, J., Edam, R., Samanipour, S., Bos, T. S., & Pirok, B. W. J. (2024). Generating realistic data through modeling and parametric probability for the numerical evaluation of data processing algorithms in two-dimensional chromatography. Analytica Chimica Acta, 1312, Article 342724. https://doi.org/10.1016/j.aca.2024.342724 [details]
- Pirok, B. W. J. (2024). Resolving Separation Issues with Computational Methods, Part 2: Why is Peak Integration Still an Issue? LCGC International, 1(6), 20-21. https://doi.org/10.56530/lcgc.int.ja6279m5 [details]
- Pirok, B. W. J. (2024). Resolving Separation Issues with Computational Methods: What Is the Retention Time, Exactly? LCGC International, 1(4), 24-25. https://doi.org/10.56530/lcgc.int.lg4875i7 [details]
- Rutan, S. C., Kempen, T., Dahlseid, T., Kruger, Z., Pirok, B., Shackman, J. G., Zhou, Y., Wang, Q., & Stoll, D. R. (2024). Improved hydrophobic subtraction model of reversed-phase liquid chromatography selectivity based on a large dataset with a focus on isomer selectivity. Journal of Chromatography A, 1731, Article 465127. https://doi.org/10.1016/j.chroma.2024.465127 [details]
- van Herwerden, D., Nikolopoulos, A., Barron, L. P., O'Brien, J. W., Pirok, B. W. J., Thomas, K. V., & Samanipour, S. (2024). Exploring the chemical subspace of RPLC: A data driven approach. Analytica Chimica Acta, 1317, Article 342869. https://doi.org/10.1016/j.aca.2024.342869 [details]
- van den Hurk, R. S., Lagerwaard, B., Terlouw, N. J., Sun, M., Tieleman, J. J., Verstegen, A. X., Samanipour, S., Pirok, B. W. J., & Gargano, A. F. G. (2024). Comprehensive Two-Dimensional Liquid Chromatography-High-Resolution Mass Spectrometry for Complex Protein Digest Analysis Using Parallel Gradients. Analytical Chemistry, 96(22), 9294-9301. https://doi.org/10.1021/acs.analchem.4c02172 [details]
- van der Zon, A. A. M., Verduin, J., van den Hurk, R. S., Gargano, A. F. G., & Pirok, B. W. J. (2024). Sample transformation in online separations: how chemical conversion advances analytical technology. Chemical Communications, 60(1), 36-50. https://doi.org/10.1039/d3cc03599a [details]
2023
- Boelrijk, J., Ensing, B., Forré, P., & Pirok, B. W. J. (2023). Closed-loop automatic gradient design for liquid chromatography using Bayesian optimization. Analytica Chimica Acta, 1242, Article 340789. https://doi.org/10.1016/j.aca.2023.340789 [details]
- Bos, T. S., Desport, J. S., Buijtenhuijs, A., Purmova, J., Karlson, L., Pirok, B. W. J., Schoenmakers, P. J., & Somsen, G. W. (2023). Composition mapping of highly substituted cellulose-ether monomers by liquid chromatography–mass spectrometry and probability-based data deconvolution. Journal of Chromatography A, 1689, Article 463758. https://doi.org/10.1016/j.chroma.2022.463758 [details]
- Kempen, T., Dahlseid, T., Lauer, T., Florea, A. C., Aase, I., Cole-Dai, N., Kaur, S., Southworth, C., Grube, K., Bhandari, J., Sylvester, M., Schimek, R., Pirok, B., Rutan, S., & Stoll, D. (2023). Characterization of a high throughput approach for large scale retention measurement in liquid chromatography. Journal of Chromatography A, 1705, Article 464182. https://doi.org/10.1016/j.chroma.2023.464182 [details]
- Knol, W. C., Peters, R. A. H., & Pirok, B. W. J. (2023). Packed modulation loops to reduce band broadening in two-dimensional liquid chromatography. Journal of Chromatography A, 1690, Article 463802. https://doi.org/10.1016/j.chroma.2023.463802 [details]
- Knol, W. C., Smeets, J. P. H., Gruendling, T., Pirok, B. W. J., & Peters, R. A. H. (2023). Online hyphenation of size-exclusion chromatography and pyrolysis-gas chromatography for polymer characterization. Journal of Chromatography A, 1690, Article 463800. https://doi.org/10.1016/j.chroma.2023.463800 [details]
- Knol, W. C., Vos, S., Gruendling, T., Pirok, B. W. J., & Peters, R. A. H. (2023). Improving the accuracy of copolymer sequence length determination by pyrolysis gas chromatography: A comprehensive study. Analytica Chimica Acta, 1238, Article 340635. https://doi.org/10.1016/j.aca.2022.340635 [details]
- Knol, W. C., de Vries, Q. L., Brooijmans, T., Gruendling, T., Pirok, B. W. J., & Peters, R. A. H. (2023). Hyphenation of liquid chromatography and pyrolysis-flame ionization detection/mass spectrometry for polymer quantification and characterization. Analytica Chimica Acta, 1257, Article 341157. https://doi.org/10.1016/j.aca.2023.341157 [details]
- Milani, N. B. L., van Gilst, E., Pirok, B. W. J., & Schoenmakers, P. J. (2023). Comprehensive two-dimensional gas chromatography: A discussion on recent innovations. Journal of Separation Science, 46(21), Article 2300304. https://doi.org/10.1002/jssc.202300304 [details]
- Molenaar, S. R. A., Bos, T. S., Boelrijk, J., Dahlseid, T. A., Stoll, D. R., & Pirok, B. W. J. (2023). Computer-driven optimization of complex gradients in comprehensive two-dimensional liquid chromatography. Journal of Chromatography A, 1707, Article 464306. https://doi.org/10.1016/j.chroma.2023.464306 [details]
- Molenaar, S. R. A., Knol, W. C., Pirok, B. W. J., & Peters, R. A. H. (2023). Development of a chemometric approach to improve the accuracy of copolymer sequence information obtained from pyrolysis gas-chromatography mass-spectrometry data. Journal of analytical and applied pyrolysis, 171, Article 105978. https://doi.org/10.1016/j.jaap.2023.105978 [details]
- Molenaar, S. R. A., Mommers, J. H. M., Stoll, D. R., Ngxangxa, S., de Villiers, A. J., Schoenmakers, P. J., & Pirok, B. W. J. (2023). Algorithm for tracking peaks amongst numerous datasets in comprehensive two-dimensional chromatography to enhance data analysis and interpretation. Journal of Chromatography A, 1705, Article 464223. https://doi.org/10.1016/j.chroma.2023.464223 [details]
- Niezen, L. E., Bos, T. S., Schoenmakers, P. J., Somsen, G. W., & Pirok, B. W. J. (2023). Capacitively coupled contactless conductivity detection to account for system-induced gradient deformation in liquid chromatography. Analytica Chimica Acta, 1271, Article 341466. https://doi.org/10.1016/j.aca.2023.341466 [details]
- Niezen, L. E., Kruijswijk, J. D., van Henten, G. B., Pirok, B. W. J., Staal, B. B. P., Radke, W., Philipsen, H. J. A., Somsen, G. W., & Schoenmakers, P. J. (2023). Principles and potential of solvent gradient size-exclusion chromatography for polymer analysis. Analytica Chimica Acta, 1253, Article 341041. https://doi.org/10.1016/j.aca.2023.341041 [details]
- Pirok, B. W. J., & Stoll, D. R. (2023). Method Development for Comprehensive Two-Dimensional Liquid Chromatography Separations. In D. R. Stoll, & P. W. Carr (Eds.), Multi-Dimensional Liquid Chromatography: Principles, Practice, and Applications (pp. 209-232). (Chromatographic Science Series). CRC Press. https://doi.org/10.1201/9781003090557-7 [details]
- Pirok, B. W. J., & Stoll, D. R. (2023). Selecting Separation Modes and Selectivities for Multi-Dimensional LC. In D. R. Stoll, & P. W. Carr (Eds.), Multi-Dimensional Liquid Chromatography: Principles, Practice, and Applications (pp. 165-182 ). (Chromatographic Science Series). CRC Press. https://doi.org/10.1201/9781003090557-5 [details]
- Pirok, B. W. J., Rutan, S. C., & Stoll, D. R. (2023). Data Analysis for Multi-Dimensional Liquid Chromatography. In D. R. Stoll, & P. W. Carr (Eds.), Multi-Dimensional Liquid Chromatography: Principles, Practice, and Applications (pp. 233-272). (Chromatographic Science Series). CRC Press. https://doi.org/10.1201/9781003090557-8 [details]
- Verduin, J., Tutiš, L., Becking, A. J., Famili, A., Zhang, K., Pirok, B. W. J., & Somsen, G. W. (2023). Characterization of Dye-Loaded Poly(lactic-co-glycolic acid) Nanoparticles by Comprehensive Two-Dimensional Liquid Chromatography Combining Hydrodynamic and Reversed-Phase Liquid Chromatography. Analytical Chemistry, 95(51), 18767-18775. https://doi.org/10.1021/acs.analchem.3c03356 [details]
- den Uijl, M. J., Bagdonaite, I., Schoenmakers, P. J., Pirok, B. W. J., & van Bommel, M. R. (2023). Incorporating a liquid-core-waveguide cell in recycling liquid chromatography for detailed studies of photodegradation reactions. Journal of Chromatography A, 1688, Article 463723. https://doi.org/10.1016/j.chroma.2022.463723 [details]
- van Henten, G. B., Bos, T. S., & Pirok, B. W. J. (2023). Approaches to Accelerate Liquid Chromatography Method Development in the Laboratory Using Chemometrics and Machine Learning. LC GC Europe, 36(6), 202–211. https://doi.org/10.56530/lcgc.eu.rh7676j5 [details]
- van Herwerden, D., O'Brien, J. W., Lege, S., Pirok, B. W. J., Thomas, K. V., & Samanipour, S. (2023). Cumulative Neutral Loss Model for Fragment Deconvolution in Electrospray Ionization High-Resolution Mass Spectrometry Data. Analytical Chemistry, 95(33), 12247-12255. https://doi.org/10.1021/acs.analchem.3c00896 [details]
- van den Hurk, R. S., Pursch, M., Stoll, D. R., & Pirok, B. W. J. (2023). Recent trends in two-dimensional liquid chromatography. TrAC - Trends in Analytical Chemistry, 166, Article 117166. https://doi.org/10.1016/j.trac.2023.117166 [details]
2022
- Bos, T. S., Boelrijk, J., Molenaar, S. R. A., Veer, B. V. ., Niezen, L. E., van Herwerden, D., Samanipour, S., Stoll, D. R., Forré, P., Ensing, B., Somsen, G. W., & Pirok, B. W. J. (2022). Chemometric Strategies for Fully Automated Interpretive Method Development in Liquid Chromatography. Analytical Chemistry, 94(46), 16060-16068. https://doi.org/10.1021/acs.analchem.2c03160 [details]
- Brau, T., Pirok, B., Rutan, S., & Stoll, D. (2022). Accuracy of retention model parameters obtained from retention data in liquid chromatography. Journal of Separation Science, 45(17), 3241-3255. https://doi.org/10.1002/jssc.202100911 [details]
- Brooijmans, T., Camoiras Gonzalez, P., Pirok, B., Schoenmakers, P., & Peters, R. (2022). Two-dimensional tools for analyzing polymer microstructure; coupling non-aqueous ion-exchange chromatography to size-exclusion chromatography. Journal of Chromatography A, 1683, Article 463536. https://doi.org/10.1016/j.chroma.2022.463536 [details]
- Brouwer, N., Maciejowska, I., McDonnell, C., Niemelä, M., Pirok, B., Grecea, Ş., Femoni, C., Ortiz-Bustos, J., Byers, W., & Wasser, I. (2022). Creating a common ground for professional development of university chemistry (STEM) lecturers in Europe. Švietimas: politika - vadyba - kokybė, 14(1), 45-57. https://doi.org/10.48127/spvk-epmq/22.14.45 [details]
- Groeneveld, I., Pirok, B. W. J., Molenaar, S. R. A., Schoenmakers, P. J., & van Bommel, M. R. (2022). The development of a generic analysis method for natural and synthetic dyes by ultra-high-pressure liquid chromatography with photo-diode-array detection and triethylamine as an ion-pairing agent. Journal of Chromatography A, 1673, Article 463038. https://doi.org/10.1016/j.chroma.2022.463038 [details]
- Knol, W. C., Gruendling, T., Schoenmakers, P. J., Pirok, B. W. J., & Peters, R. A. H. (2022). Co-Polymer sequence determination over the molar mass distribution by size-exclusion chromatography combined with pyrolysis - gas chromatography. Journal of Chromatography A, 1670, Article 462973. https://doi.org/10.1016/j.chroma.2022.462973 [details]
- Knol, W. C., Vos, S., Gruendling, T., Pirok, B. W. J., & Peters, R. A. H. (2022). Expansion of the application range of pyrolysis-gas chromatography to copolymer sequence determination: Acrylate copolymers. Journal of analytical and applied pyrolysis, 165, Article 105578. https://doi.org/10.1016/j.jaap.2022.105578 [details]
- Molenaar, S. R. A., Savova, M., Cross, R., Ferguson, P. D., Schoenmakers, P. J., & Pirok, B. W. J. (2022). Improving retention-time prediction in supercritical-fluid chromatography by multivariate modelling. Journal of Chromatography A, 1668, Article 462909. https://doi.org/10.1016/j.chroma.2022.462909 [details]
- Molenaar, S. R. A., van de Put, B., Desport, J. S., Samanipour, S., Peters, R. A. H., & Pirok, B. W. J. (2022). Automated Feature Mining for Two-Dimensional Liquid Chromatography Applied to Polymers Enabled by Mass Remainder Analysis. Analytical Chemistry, 94(14), Article 5599-5607. https://doi.org/10.1021/acs.analchem.1c05336 [details]
- Niezen, L. E., Schoenmakers, P. J., & Pirok, B. W. J. (2022). Critical comparison of background correction algorithms used in chromatography. Analytica Chimica Acta, 1201, Article 339605. https://doi.org/10.1016/j.aca.2022.339605 [details]
- Niezen, L. E., Staal, B. B. P., Lang, C., Philipsen, H. J. A., Pirok, B. W. J., Somsen, G. W., & Schoenmakers, P. J. (2022). Recycling gradient-elution liquid chromatography for the analysis of chemical-composition distributions of polymers. Journal of Chromatography A, 1679, Article 463386. https://doi.org/10.1016/j.chroma.2022.463386 [details]
- Stoll, D. R., Kainz, G., Dahlseid, T. A., Kempen, T. J., Brau, T., & Pirok, B. W. J. (2022). An approach to high throughput measurement of accurate retention data in liquid chromatography. Journal of Chromatography A, 1678, Article 463350. https://doi.org/10.1016/j.chroma.2022.463350 [details]
- den Uijl, M. J., Lokker, A., van Dooren, B., Schoenmakers, P. J., Pirok, B. W. J., & van Bommel, M. R. (2022). Comparing different light-degradation approaches for the degradation of crystal violet and eosin Y. Dyes and Pigments, 197, Article 109882. https://doi.org/10.1016/j.dyepig.2021.109882 [details]
- den Uijl, M. J., Roeland, T., Bos, T. S., Schoenmakers, P. J., van Bommel, M. R., & Pirok, B. W. J. (2022). Assessing the feasibility of stationary-phase-assisted modulation for two-dimensional liquid-chromatography separations. Journal of Chromatography A, 1679, Article 463388. https://doi.org/10.1016/j.chroma.2022.463388 [details]
- den Uijl, M. J., Van Der Wijst, Y. J. H. L., Groeneveld, I., Schoenmakers, P. J., Pirok, B. W. J., & Van Bommel, M. R. (2022). Combining Photodegradation in a Liquid-Core-Waveguide Cell with Multiple-Heart-Cut Two-Dimensional Liquid Chromatography. Analytical Chemistry, 94(31), 11055-11061. https://doi.org/10.1021/acs.analchem.2c01928 [details]
- van den Hurk, R. S., Abdulhussain, N., van beurden, A., Dekker, M., Hulsbergen, A., Peters, R. A. H., Pirok, B. W. J., & van Asten, A. C. (2022). Characterization and comparison of smokeless powders by on-line two-dimensional liquid chromatography. Journal of Chromatography A, 1672, Article 463072. https://doi.org/10.1016/j.chroma.2022.463072 [details]
2021
- Boelrijk, J., Pirok, B., Ensing, B., & Forré, P. (2021). Bayesian optimization of comprehensive two-dimensional liquid chromatography separations. Journal of Chromatography A, 1659, Article 462628. https://doi.org/10.1016/j.chroma.2021.462628 [details]
- Bos, T. S., Niezen, L. E., den Uijl, M. J., Molenaar, S. R. A., Lege, S., Schoenmakers, P. J., Somsen, G. W., & Pirok, B. W. J. (2021). Reducing the influence of geometry-induced gradient deformation in liquid chromatographic retention modelling. Journal of Chromatography A, 1635, Article 461714. https://doi.org/10.1016/j.chroma.2020.461714 [details]
- Knol, W. C., Pirok, B. W. J., & Peters, R. A. H. (2021). Detection challenges in quantitative polymer analysis by liquid chromatography. Journal of Separation Science, 44(1), 63-87. https://doi.org/10.1002/jssc.202000768 [details]
- Lazaridi, E., Janssen, H.-G., Vincken, J.-P., Pirok, B., & Hennebelle, M. (2021). A comprehensive two-dimensional liquid chromatography method for the simultaneous separation of lipid species and their oxidation products. Journal of Chromatography A, 1644, Article 462106. https://doi.org/10.1016/j.chroma.2021.462106 [details]
- Molenaar, S. R. A., Dahlseid, T. A., Leme, G. M., Stoll, D. R., Schoenmakers, P. J., & Pirok, B. W. J. (2021). Peak-tracking algorithm for use in comprehensive two-dimensional liquid chromatography-Application to monoclonal-antibody peptides. Journal of Chromatography A, 1639, Article 461922. https://doi.org/10.1016/j.chroma.2021.461922 [details]
- Niezen, L. E., Staal, B. B. P., Lang, C., Pirok, B. W. J., & Schoenmakers, P. J. (2021). Thermal modulation to enhance two-dimensional liquid chromatography separations of polymers. Journal of Chromatography A, 1653, Article 462429. https://doi.org/10.1016/j.chroma.2021.462429 [details]
- Pirok, B. W. J., Mengerink, Y., & Peters, R. A. H. (2021). Molecular Correlative Material Characterization: Advantages for Polymer Analysis Using Liquid Chromatography. LC GC Europe, 34(5), 172-180. https://www.chromatographyonline.com/view/molecular-correlative-material-characterization-advantages-for-polymer-analysis-using-liquid-chromatography [details]
- Samanipour, S., Choi, P., O'Brien, J. W., Pirok, B. W. J., Reid, M. J., & Thomas, K. V. (2021). From Centroided to Profile Mode: Machine Learning for Prediction of Peak Width in HRMS Data. Analytical Chemistry, 93(49), 16562–16570. https://doi.org/10.1021/acs.analchem.1c03755 [details]
- den Uijl, M. J., Schoenmakers, P. J., Pirok, B. W. J., & van Bommel, M. R. (2021). Recent applications of retention modelling in liquid chromatography. Journal of Separation Science, 44(1), 88-114. https://doi.org/10.1002/jssc.202000905 [details]
- den Uijl, M. J., Schoenmakers, P. J., Schulte, G. K., Stoll, D. R., van Bommel, M. R., & Pirok, B. W. J. (2021). Measuring and using scanning-gradient data for use in method optimization for liquid chromatography. Journal of Chromatography A, 1636, Article 461780. https://doi.org/10.1016/j.chroma.2020.461780 [details]
2020
- Aly, A. A., Muller, M., de Villiers, A., Pirok, B. W. J., & Górecki, T. (2020). Parallel gradients in comprehensive multidimensional liquid chromatography enhance utilization of the separation space and the degree of orthogonality when the separation mechanisms are correlated. Journal of Chromatography A, 1628, Article 461452. https://doi.org/10.1016/j.chroma.2020.461452 [details]
- Bos, T. S., Knol, W. C., Molenaar, S. R. A., Niezen, L. E., Schoenmakers, P. J., Somsen, G. W., & Pirok, B. W. J. (2020). Recent applications of chemometrics in one- and two-dimensional chromatography. Journal of Separation Science, 43(9-10), 1678-1727. https://doi.org/10.1002/jssc.202000011 [details]
- Pirok, B. W. J., & Westerhuis, J. A. (2020). Challenges in Obtaining Relevant Information from One- and Two-Dimensional LC Experiments. Supplement to LCGC North America solutions for seperation scientists, 6(38), 8, 10-14. https://doi.org/10.56530/lcgc.na.jk4782s5 [details]
- van Herwerden, D., Pirok, B. W. J., & Schoenmakers, P. J. (2020). Liquid Chromatography: Applications for the Oil and Gas Industry. In M. N. Dunkle, & W. L. Winniford (Eds.), Analytical Techniques in the Oil and Gas Industry for Environmental Monitoring (pp. 225-258). Wiley. https://doi.org/10.1002/9781119523314.ch5 [details]
2019
- Brooijmans, T., Okhuijsen, R. A., Oerlemans, G. M. M., Pirok, B. W. J., Schoenmakers, P. J., & Peters, R. A. H. (2019). Heterogeneity analysis of polymeric carboxylic acid functionality by selective derivatization followed by size exclusion chromatography. Analytica Chimica Acta, 1072, 87-94. https://doi.org/10.1016/j.aca.2019.04.051 [details]
- Groeneveld, G., Pirok, B. W. J., & Schoenmakers, P. J. (2019). Perspectives on the future of multi-dimensional platforms. Faraday Discussions, 218, 72-100. https://doi.org/10.1039/c8fd00233a [details]
- Haselberg, R., Pirok, B. W. J., Gargano, A. F. G., & Kohler, I. (2019). Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques. LC GC Europe, 32(9), 465-480. https://doi.org/10.56530/lcgc.eu.ry4267t3 [details]
- Haselberg, R., Pirok, B. W. J., Gargano, A. F. G., & Kohler, I. (2019). Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques. LC-GC North America, 37(12), 878-887. https://doi.org/10.56530/lcgc.eu.ry4267t3 [details]
- Pirok, B. W. J., Abdulhussain, N., Brooijmans, T., Nabuurs, T., de Bont, J., Schellekens, M. A. J., Peters, R. A. H., & Schoenmakers, P. J. (2019). Analysis of charged acrylic particles by on-line comprehensive two-dimensional liquid chromatography and automated data-processing. Analytica Chimica Acta, 1054, 184-192. https://doi.org/10.1016/j.aca.2018.12.059 [details]
- Pirok, B. W. J., Moro, G., Meekel, N., Berbers, S. V. J., Schoenmakers, P. J., & van Bommel, M. R. (2019). Mapping degradation pathways of natural and synthetic dyes with LC-MS: Influence of solvent on degradation mechanisms. Journal of Cultural Heritage, 38, 29-36. https://doi.org/10.1016/j.culher.2019.01.003 [details]
- Pirok, B. W. J., Stoll, D. R., & Schoenmakers, P. J. (2019). Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical Applications. Analytical Chemistry, 91(1), 240-263. https://doi.org/10.1021/acs.analchem.8b04841 [details]
- Pirok, B. W. J., den Uijl, M. J., Moro, G., Berbers, S. V. J., Croes, C. J. M., van Bommel, M. R., & Schoenmakers, P. J. (2019). Characterization of Dye Extracts from Historical Cultural-Heritage Objects using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients. Analytical Chemistry, 91(4), 3062-3069. https://doi.org/10.1021/acs.analchem.8b05469 [details]
- Roca, L. S., Schoemaker, S. E., Pirok, B. W. J., Gargano, A. F. G., & Schoenmakers, P. J. (2019). Accurate modelling of the retention behaviour of peptides in gradient-elution hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1614, Article 460650. https://doi.org/10.1016/j.chroma.2019.460650 [details]
- Wouters, B., Pirok, B. W. J., Soulis, D., Garmendia Perticarini, R. C., Fokker, S., van den Hurk, R. S., Skolimowski, M., Peters, R. A. H., & Schoenmakers, P. J. (2019). On-line microfluidic immobilized-enzyme reactors: A new tool for characterizing synthetic polymers. Analytica Chimica Acta, 1053, 62-69. https://doi.org/10.1016/j.aca.2018.12.002 [details]
- van Schaick, G., Pirok, B. W. J., Haselberg, R., Somsen, G. W., & Gargano, A. F. G. (2019). Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms. Journal of Chromatography A, 1598, 67-76. https://doi.org/10.1016/j.chroma.2019.03.038 [details]
2018
- Pirok, B. W. J., & Schoenmakers, P. J. (2018). Practical Approaches to Overcome the Challenges of Comprehensive Two-Dimensional Liquid Chromatography. LC GC Europe, 31(5), 242, 244, 246, 248-249. http://www.chromatographyonline.com/practical-approaches-overcome-challenges-comprehensive-two-dimensional-liquid-chromatography [details]
- Pirok, B. W. J., Gargano, A. F. G., & Schoenmakers, P. J. (2018). Optimizing separations in online comprehensive two-dimensional liquid chromatography. Journal of Separation Science, 41(1), 68-98. https://doi.org/10.1002/jssc.201700863 [details]
- Pirok, B. W. J., Molenaar, S. R. A., Roca, L. S., & Schoenmakers, P. J. (2018). Peak-Tracking Algorithm for Use in Automated Interpretive Method-Development Tools in Liquid Chromatography. Analytical Chemistry, 90(23), 14011-14019. https://doi.org/10.1021/acs.analchem.8b03929 [details]
- van Beek, F. T., Edam, R., Pirok, B. W. J., Genuit, W. J. L., & Schoenmakers, P. J. (2018). Comprehensive two-dimensional liquid chromatography of heavy oil. Journal of Chromatography A, 1564, 110-119. https://doi.org/10.1016/j.chroma.2018.06.001 [details]
2017
- Pirok, B. W. J., Abdulhussain, N., Aalbers, T., Wouters, B., Peters, R. A. H., & Schoenmakers, P. J. (2017). Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation. Analytical Chemistry, 89(17), 9167-9174. https://doi.org/10.1021/acs.analchem.7b01906 [details]
- Pirok, B. W. J., Breuer, P., Hoppe, S. J. M., Chitty, M., Welch, E., Farkas, T., van der Wal, S., Peters, R., & Schoenmakers, P. J. (2017). Size-exclusion chromatography using core-shell particles. Journal of Chromatography A, 1486, 96-102. https://doi.org/10.1016/j.chroma.2016.12.015 [details]
- Pirok, B. W. J., Molenaar, S. R. A., van Outersterp, R. E., & Schoenmakers, P. J. (2017). Applicability of retention modelling in hydrophilic-interaction liquid chromatography for algorithmic optimization programs with gradient-scanning techniques. Journal of Chromatography A, 1530, 104-111. https://doi.org/10.1016/j.chroma.2017.11.017 [details]
2016
- Pirok, B. W. J., Knip, J., van Bommel, M. R., & Schoenmakers, P. J. (2016). Characterization of synthetic dyes by comprehensive two-dimensional liquid chromatography combining ion-exchange chromatography and fast ion-pair reversed-phase chromatography. Journal of Chromatography A, 1436, 141-146. https://doi.org/10.1016/j.chroma.2016.01.070 [details]
- Pirok, B. W. J., Pous-Torres, S., Ortiz-Bolsico, C., Vivó-Truyols, G., & Schoenmakers, P. J. (2016). Program for the interpretive optimization of two-dimensional resolution. Journal of Chromatography A, 1450, 29-37. https://doi.org/10.1016/j.chroma.2016.04.061 [details]
2020
- den Uijl, M. J., Mai, D. X., Eskildsen, C. E. A., de Voogt, P., Pirok, B. W. J., Schoenmakers, P. J., & van Bommel, M. R. (2020). Mapping degradation pathways of pharmaceuticals in advanced oxidation with liquid chromatography - UV/MS. Poster session presented at SETAC SciCon.
- den Uijl, M. J., Schoenmakers, P. J., Schulte, G. K., Stoll, D. R., van Bommel, M. R., & Pirok, B. W. J. (2020). Measuring and using scanning-gradient data for LC. Poster session presented at CHAINS 2020.
2019
- den Uijl, M. J., Groeneveld, I., Pirok, B. W. J., Ariese, F., Somsen, G. W., Schoenmakers, P. J., & van Bommel, M. R. (2019). Toolbox for studying the chemistry of light induced degradation (TOOCOLD): towards an online degradation system. Poster session presented at HPLC 2019, Milan, Italy.
- den Uijl, M. J., Pirok, B. W. J., de Voogt, W. P., Schoenmakers, P. J., & van Bommel, M. R. (2019). Mapping degradation pathways in advanced oxidation processes with two-dimensional liquid chromatography with an online light-degradation system. Poster session presented at SETAC Europe 29th annual meeting, Helsinki, Finland.
2018
- Berbers, S., Pirok, B. W. J., Moro, G., Croes, C., Knip, J., Meekel, N., van Bommel, M. R., & Schoenmakers, P. J. (2018). Natural and Synthetic Dye Characterization by Comprehensive Two Dimensional Liquid Chromatography. Poster session presented at Gordon Research Conference on Scientific Methods in Cultural Heritage Research, Barcelona, Spain.
Prize / grant
- Pirok, B. (2025). STREAMLINED: Improving analytical science to get a grip on nanoplastics. https://hims.uva.nl/content/news/2025/07/streamlined-improving-analytical-science-to-get-a-grip-on-nanoplastics.html?origin=ZBsmRfebSyq59D2IosWhGQ
- Noël, T., Gargano, A. & Pirok, B. (2024). MMD hub grant: MAP-RoboBioConjugator: Molecular Analytics Platform for the Robotic BioConjugation chemistry self-optimization processes. https://hims.uva.nl/shared/faculteiten/en/faculteit-der-natuurwetenschappen-wiskunde-en-informatica/news/2024/07/nine-research-projects-chos…
- Pirok, B. (2024). 2024 HTC Innovation Award. https://hims.uva.nl/content/news/2024/06/bob-pirok-wins-2024-htc-innovation-award.html?origin=4zNaIwCsT1iMc%2FL4U9Sh2Q
- van Bommel, M., Samanipour, S. & Pirok, B. (2024). Controlled Study Of Light Induced Degradation by Advancing Technology (ConSOLIDATe). https://hims.uva.nl/content/news/2024/12/nwo-funding-for-advanced-study-of-light-induced-materials-degradation.html
- Pirok, B. (2022). Veni grant for Bob Pirok. https://hims.uva.nl/content/news/2022/04/veni-grant-for-analytical-scientist-bob-pirok.html?origin=4zNaIwCsT1iMc%2FL4U9Sh2Q
- Gargano, A. & Pirok, B. (2022). ‘Top 40 Under 40’ of analytical scientists worldwide. https://hims.uva.nl/content/news/2022/10/andrea-gargano-and-bob-pirok-featured-in-top-40-under-40-of-analytical-scientists-worldwide.html?or…
- Somsen, G., Rijs, A., Pirok, B. & Gargano, A. (2022). Funding for research into analysis of oligonucleotide therapeutics. https://hims.uva.nl/content/news/2022/05/funding-for-research-into-analysis-of-oligonucleotide-therapeutics.html?origin=4zNaIwCsT1iMc%2FL4U9Sh2Q
- Schoenmakers, P. & Pirok, B. (2021). Two UvA analytical scientists in worldwide 'Power List'.
- van Asten, A., Peters, R., Schoenmakers, P. & Pirok, B. (2020). NWO funding for PARADISE. https://hims.uva.nl/content/news/2020/02/paradise-bringing-analytical-science-to-the-next-level.html?origin=e9Lg9H1kRbuEFvhk7SGgCg
- Pirok, B. (2019). Tenure track position for analytical chemist Bob Pirok. https://hims.uva.nl/content/news/2019/04/tenure-track-position-for-analytical-chemist-bob-pirok.html
- Pirok, B. W. J. (2018). Journal of Chromatography A Young Scientist Award.
- Pirok, B. (2017). Csaba Horváth Award. http://hims.uva.nl/content/news/2017/06/bob-pirok-wins-csaba-horvath-young-scientist-award-2017.html
- Pirok, B. (2015). Shimadzu Young Scientist Award. http://hims.uva.nl/news/content/2015/10/analytical-chemist-bob-pirok-wins-young-scientist-award.html
Membership / relevant position
- Pirok, B. (2022-). Member of ACS (American Chemical Society), American Chemical Society.
- Pirok, B. (2022-). Member of the European Chemistry Thematic Network, European Chemistry Thematic Network .
- Pirok, B. (2021-). Early Career Board Member of ACS Analytical Chemistry, American Chemical Society.
Media appearance
- Pirok, B. (03-06-2025). HPLC2025: de zin en onzin van AI [Web] C2W. Bob Pirok in C2W on AI. https://www.sciencelink.net/nieuws/hplc2025-de-zin-en-onzin-van-ai/22733.article
- Pirok, B. (30-11-2021). Musings from the Power List: Bob Pirok the Analytical Scientist. Musings from the Power List: Bob Pirok. https://theanalyticalscientist.com/business-education/musings-from-the-power-list-bob-pirok
- den Uijl, M., Schoenmakers, P., Pirok, B. & van Bommel, M. (27-01-2021). Recent applications of retention modelling in liquid chromatography [Web]. Recent applications of retention modelling in liquid chromatography. https://www.uva.nl/en/shared-content/subsites/van-t-hoff-institute-for-molecular-sciences/en/news/2021/01/videopitch-recent-applications-of-…
- Pirok, B. (17-12-2020). How Algorithms Are Revolutionizing Liquid Chromatography Technology Networks. How Algorithms Are Revolutionizing Liquid Chromatography. https://www.technologynetworks.com/informatics/blog/insight-how-algorithms-are-revolutionizing-liquid-chromatography-with-dr-bob-pirok-344102
Talk / presentation
- Pirok, B. (speaker) (21-10-2024). Automating Method Development for 1D and 2D Liquid Chromatography, 53rd International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques, Dalian.
- van Henten, G. (speaker), Bos, T. (speaker) & Pirok, B. (speaker) (8-10-2024). Automating Method Development for 1D and 2D-LC of Biopharmaceuticals: A Roadmap, 34th International Symposium on Chromatography – ISC 2024) - ISC2024, Liverpool.
- Pirok, B. (speaker) (24-7-2024). Crossing the (Correct) Dams: Automating LC Method Development and the Role of Optimization Algorithms, 52nd International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques , Denver.
- Pirok, B. (speaker) (27-6-2024). The Role of Peak Detection in Realizing Automation of Method Development, Nouvelles tendances en Sciences Séparatives (chimie verte, automatisation, miniaturisation, impression 3D, IA).
- Pirok, B. (speaker) (5-6-2024). Improving the Sustainability of Liquid Chromatography using Machine Learning, 28th Annual Green Chemistry & Engineering Conference (GC&E 2024), Atlanta.
- Pirok, B. (speaker) (31-5-2024). Making Separation Technology more Accessible, 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-18), Leuven.
- van Bommel, M. (speaker), Groeneveld, I. (speaker), den Uijl, M. (speaker), Pirok, B. (speaker), Ariese, F. (speaker), Somsen, G. W. (speaker) & Schoenmakers, P. (speaker) (2024). TooCOLD: Toolbox for studying the Chemistry Of Light-induced Degradation, a review, 43 annual meeting of Dyes in History & Archaeology, Leeds.
- Pirok, B. (speaker) (21-3-2023). Challenges to Achieve Unsupervised Optimization of Heart-cut and Comprehensive Two-dimensional Liquid Chromatography Separations, Pittsburgh Conference 2023, Philadelphia, PA, United States, Philadelphia.
- Pirok, B. (speaker) (23-8-2022). Closed-loop automated method development in (2D) liquid chromatography using retention modelling and machine learning, ACS Fall 2022, Chicago.
- Pirok, B. (speaker) (21-6-2022). Unsupervised Method Development for Liquid Chromatography, HPLC2022, San Diego, CA, United States, San Diego.
- Pirok, B. (speaker) (14-9-2021). Multi-Dimensional Techniques - Theoretical and Practical Rationale for 2D-LC, Instrumentation, Modulation and Method Development, Community for Analytical Measurement Science Conference 2022.
- Pirok, B. (speaker) (7-11-2019). Using 2D-LC: from small molecules to large particles, Discussiegroep Scheidingsmethoden voor Polymeren Symposium Herfst 2019, Breda.
- Pirok, B. (speaker) (29-10-2019). Opportunities for Robust Application of Two-dimensional Liquid Chromatography, Chromatographic Society Multi-dimensional Chromatography Symposium, Bracknell.
- Pirok, B. (speaker) (26-6-2019). Opportunities in Two-dimensional Liquid Chromatography through PARADISE, Agilent University Relations Fair 2019, Waldbronn.
- Pirok, B. (speaker) (21-5-2019). 2D-LC: From data analysis to method development, Forum for Analytical Science and Technology, Veldhoven.
- Pirok, B. (speaker) (2019). Computer-optimized LC×LC-MS for the Characterization of 17th-century Cultural Heritage Objects, 48th International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques, Milan.
- Pirok, B. (speaker) (2019). Peak Tracking for Multi-dimensional Chromatography, 12th Balaton Symposium on High-Performance Separation Methods, Balaton.
- Pirok, B. (speaker) (2019). Analysis of Polymeric Particles, 9th International Symposium on the Separation and Characterization of Natural and Synthetic Macromolecules, Amsterdam.
- Pirok, B. (speaker) (2019). Enhancing method development using peak-tracking algorithms to rapidly interpret data, 49th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Kyoto.
- Pirok, B. (speaker) (2019). Rapid and Effective Implementation of Comprehensive 2D-LC for 'Impossible' Separations, The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Philadelphia.
- Groeneveld, I. (speaker), Pirok, B. (speaker), Schoenmakers, P. (speaker) & van Bommel, M. (speaker) (25-10-2018). One size fits all – one method for the simultaneous analysis of natural and synthetic dyes by UHPLC-PDA (optimised by PIOTR), Dyes in History and Archaeology, Lisbon.
- Pirok, B. (speaker), Moro, G. (speaker), Meekel, N. (speaker), Berbers, S. (speaker), Schoenmakers, P. (speaker) & van Bommel, M. (speaker) (25-10-2018). Mapping degradation pathways of natural and synthetic dyes with LC-MS: influence of solvent on the degradation of eosin and carminic acid, Dyes in History and Archaeology, Lisbon.
- Pirok, B. W. J. (speaker) (3-10-2017). Nanoparticle Analysis by Actively Modulated HDC×SEC with Intermediate Sample Transformation, Labtechnology Conference 2017, Utrecht.
Others
- Pirok, B. (participant), Grecea, S. (participant), Schreuders, L. (participant) & Brouwer, N. (participant) (10-10-2021 - 15-10-2021). STEM-CPD Summer School. To guarantee that teaching and learning opportunities provided to students are of high quality, the European Chemistry Thematic Network (ECTN) and (…) (participating in a conference, workshop, ...). https://hims.uva.nl/content/news/2021/10/lecturers-participated-in-first-stem-cpd-summer-school.html?origin=4zNaIwCsT1iMc%2FL4U9Sh2Q
2025
2024
2023
- Knol, W. C. (2023). Pyrolysis-based methods for polymer characterization. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Molenaar, S. R. A. (2023). Strategies for automated chromatographic method development and data interpretation. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- Niezen, L. E. (2023). New methods for the characterization of essential distributions. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
2022
- Brooijmans, T. (2022). Specific separation- and detection strategies for the characterization of acid-functional polymers. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
- den Uijl, M. J. (2022). Under the influence of light: New chromatographic tools for elucidating photodegradation mechanisms. [Thesis, fully internal, Universiteit van Amsterdam]. [details]
This list of publications is extracted from the UvA-Current Research Information System. Questions? Ask the library or the Pure staff of your faculty / institute. Log in to Pure to edit your publications. Log in to Personal Page Publication Selection tool to manage the visibility of your publications on this list. -
Ancillary activities
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Royal Society of Chemistry
Schrijven van het leerboek "Analytical Separation Sciences". -
Gustavus Adolphus College
Visiting Research Professor
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Royal Society of Chemistry
van Henten, G. B., Boelrijk, J., Kattenberg, C., Bos, T. S., Ensing, B., Forré, P., & Pirok, B. W. J. (2025). Comparison of optimization algorithms for automated method development of gradient profiles. Journal of Chromatography A, 1742, Article 465626.