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dr. B.W.J. (Bob) Pirok

Faculty of Science
Van 't Hoff Institute for Molecular Sciences
Photographer: B.E. de Jong

Visiting address
  • Science Park 904
  • Room number:C2.258
Postal address
  • Postbus 94157
    1090 GD Amsterdam
Contact details
  • Publications


    • Bos, T. S., Knol, W. C., Molenaar, S. R. A., Niezen, L. E., Schoenmakers, P. J., Somsen, G. W., & Pirok, B. W. J. (2020). Recent applications of chemometrics in one- and two-dimensional chromatography. Journal of Separation Science, 43(9-10, SI), 1678-1727.


    • Brooijmans, T., Okhuijsen, R. A., Oerlemans, G. M. M., Pirok, B. W. J., Schoenmakers, P. J., & Peters, R. A. H. (2019). Heterogeneity analysis of polymeric carboxylic acid functionality by selective derivatization followed by size exclusion chromatography. Analytica Chimica Acta, 1072, 87-94. [details]
    • Groeneveld, G., Pirok, B. W. J., & Schoenmakers, P. J. (2019). Perspectives on the future of multi-dimensional platforms. Faraday Discussions, 218, 72-100. [details]
    • Haselberg, R., Pirok, B. W. J., Gargano, A. F. G., & Kohler, I. (2019). Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques. LC GC Europe, 32(9), 465-480. [details]
    • Pirok, B. W. J., Abdulhussain, N., Brooijmans, T., Nabuurs, T., de Bont, J., Schellekens, M. A. J., ... Schoenmakers, P. J. (2019). Analysis of charged acrylic particles by on-line comprehensive two-dimensional liquid chromatography and automated data-processing. Analytica Chimica Acta, 1054, 184-192. [details]
    • Pirok, B. W. J., Moro, G., Meekel, N., Berbers, S. V. J., Schoenmakers, P. J., & van Bommel, M. R. (2019). Mapping degradation pathways of natural and synthetic dyes with LC-MS: Influence of solvent on degradation mechanisms. Journal of Cultural Heritage, 38, 29-36. [details]
    • Pirok, B. W. J., Stoll, D. R., & Schoenmakers, P. J. (2019). Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical Applications. Analytical Chemistry, 91(1), 240-263. [details]
    • Pirok, B. W. J., den Uijl, M. J., Moro, G., Berbers, S. V. J., Croes, C. J. M., van Bommel, M. R., & Schoenmakers, P. J. (2019). Characterization of Dye Extracts from Historical Cultural-Heritage Objects using State-of-the-Art Comprehensive Two-Dimensional Liquid Chromatography and Mass Spectrometry with Active Modulation and Optimized Shifting Gradients. Analytical Chemistry, 91(4), 3062-3069. [details]
    • Roca, L. S., Schoemaker, S. E., Pirok, B. W. J., Gargano, A. F. G., & Schoenmakers, P. J. (2019). Accurate modelling of the retention behaviour of peptides in gradient-elution hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1614, [460650]. [details]
    • Wouters, B., Pirok, B. W. J., Soulis, D., Garmendia Perticarini, R. C., Fokker, S., van den Hurk, R. S., ... Schoenmakers, P. J. (2019). On-line microfluidic immobilized-enzyme reactors: A new tool for characterizing synthetic polymers. Analytica Chimica Acta, 1053, 62-69. [details]
    • van Schaick, G., Pirok, B. W. J., Haselberg, R., Somsen, G. W., & Gargano, A. F. G. (2019). Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms. Journal of Chromatography A, 1598, 67-76. [details]



    • Pirok, B. W. J., Abdulhussain, N., Aalbers, T., Wouters, B., Peters, R. A. H., & Schoenmakers, P. J. (2017). Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation. Analytical Chemistry, 89(17), 9167-9174. [details]
    • Pirok, B. W. J., Breuer, P., Hoppe, S. J. M., Chitty, M., Welch, E., Farkas, T., ... Schoenmakers, P. J. (2017). Size-exclusion chromatography using core-shell particles. Journal of Chromatography A, 1486, 96-102. [details]
    • Pirok, B. W. J., Molenaar, S. R. A., van Outersterp, R. E., & Schoenmakers, P. J. (2017). Applicability of retention modelling in hydrophilic-interaction liquid chromatography for algorithmic optimization programs with gradient-scanning techniques. Journal of Chromatography A, 1530, 104-111. [details]


    • Pirok, B. W. J., Knip, J., van Bommel, M. R., & Schoenmakers, P. J. (2016). Characterization of synthetic dyes by comprehensive two-dimensional liquid chromatography combining ion-exchange chromatography and fast ion-pair reversed-phase chromatography. Journal of Chromatography A, 1436, 141-146. [details]
    • Pirok, B. W. J., Pous-Torres, S., Ortiz-Bolsico, C., Vivó-Truyols, G., & Schoenmakers, P. J. (2016). Program for the interpretive optimization of two-dimensional resolution. Journal of Chromatography A, 1450, 29-37. [details]


    • den Uijl, M. J., Groeneveld, I., Pirok, B. W. J., Ariese, F., Somsen, G. W., Schoenmakers, P. J., & van Bommel, M. R. (2019). Toolbox for studying the chemistry of light induced degradation (TOOCOLD): towards an online degradation system. Poster session presented at HPLC 2019, Milan, Italy.
    • den Uijl, M. J., Pirok, B. W. J., de Voogt, W. P., Schoenmakers, P. J., & van Bommel, M. R. (2019). Mapping degradation pathways in advanced oxidation processes with two-dimensional liquid chromatography with an online light-degradation system. Poster session presented at SETAC Europe 29th annual meeting, Helsinki, Finland.


    • Berbers, S., Pirok, B. W. J., Moro, G., Croes, C., Knip, J., Meekel, N., ... Schoenmakers, P. J. (2018). Natural and Synthetic Dye Characterization by Comprehensive Two Dimensional Liquid Chromatography. Poster session presented at Gordon Research Conference on Scientific Methods in Cultural Heritage Research, Barcelona, Spain.


    • Pirok, B. (2019). Tenure track position for analytical chemist Bob Pirok.
    • Pirok, B. W. J. (2018). Journal of Chromatography A Young Scientist Award.
    • Pirok, B. (2017). Csaba Horváth Award.
    • Pirok, B. (2015). Shimadzu Young Scientist Award.

    Talk / presentation

    • Pirok, B. (speaker) (7-11-2019). Using 2D-LC: from small molecules to large particles, Discussiegroep Scheidingsmethoden voor Polymeren Symposium Herfst 2019, Breda.
    • Pirok, B. (speaker) (29-10-2019). Opportunities for Robust Application of Two-dimensional Liquid Chromatography, Chromatographic Society Multi-dimensional Chromatography Symposium, Bracknell.
    • Pirok, B. (speaker) (26-6-2019). Opportunities in Two-dimensional Liquid Chromatography through PARADISE, Agilent University Relations Fair 2019, Waldbronn.
    • Pirok, B. (speaker) (21-5-2019). 2D-LC: From data analysis to method development, Forum for Analytical Science and Technology, Veldhoven.
    • Pirok, B. (speaker) (2019). Computer-optimized LC×LC-MS for the Characterization of 17th-century Cultural Heritage Objects, 48th International Symposium on High-Performance-Liquid-Phase Separations and Related Techniques, Milan.
    • Pirok, B. (speaker) (2019). Peak Tracking for Multi-dimensional Chromatography, 12th Balaton Symposium on High-Performance Separation Methods, Balaton.
    • Pirok, B. (speaker) (2019). Analysis of Polymeric Particles, 9th International Symposium on the Separation and Characterization of Natural and Synthetic Macromolecules, Amsterdam.
    • Pirok, B. (speaker) (2019). Enhancing method development using peak-tracking algorithms to rapidly interpret data, 49th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Kyoto.
    • Pirok, B. (speaker) (2019). Rapid and Effective Implementation of Comprehensive 2D-LC for 'Impossible' Separations, The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy, Philadelphia.
    • Groeneveld, I. (speaker), Pirok, B. (speaker), Schoenmakers, P. (speaker) & van Bommel, M. (speaker) (25-10-2018). One size fits all – one method for the simultaneous analysis of natural and synthetic dyes by UHPLC-PDA (optimised by PIOTR), Dyes in History and Archaeology, Lisbon.
    • Pirok, B. (speaker), Moro, G. (speaker), Meekel, N. (speaker), Berbers, S. (speaker), Schoenmakers, P. (speaker) & van Bommel, M. (speaker) (25-10-2018). Mapping degradation pathways of natural and synthetic dyes with LC-MS: influence of solvent on the degradation of eosin and carminic acid, Dyes in History and Archaeology, Lisbon.
    • Pirok, B. W. J. (speaker) (3-10-2017). Nanoparticle Analysis by Actively Modulated HDC×SEC with Intermediate Sample Transformation, Labtechnology Conference 2017, Utrecht.


    • Pirok, B. W. J. (2019). Making ANalytically Incompatible Approaches Compatible. [details]
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  • Ancillary activities
    • Gustavus Adolphus College
      Visiting Research Professor